EC05 Coprinus comatus cultivation techniques

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1. The parent species, parent species, and cultivar of the strain were selected as full-nutrition, high-nitrogen type strains, and the universal medium was prepared as a one-time formulation. The mother culture medium was filled with 18180 mm glass test tubes, sterilized in an autoclave with an inlet diameter of 22 to 24 cm for 40 minutes, and the culture medium of the original species and cultivars was filled with 500 ml cans or 500 ml saline bottles and placed in space. Bacteria (self-made material costs about 10 yuan, can hold 500 to 800 bottles) sterilization within 3 to 4 hours, or placed in an atmospheric sterilization pot (upper steamer) sterilization within 8 to 10 hours, after cooling to room temperature take out. Inoculation with a new open type sterile mushroom inoculum (self-produced material costs 3 to 5 yuan), the above-mentioned culture medium directly on the inoculator operating platform, random mother, the original species, cultivars full open process Expanding and transplanting to inoculate. After inoculation, set at 1.5 ~ 28 °C in a clean environment shading culture, generally 8 to 12 days mycelium can be filled with culture containers, shorter cycle than conventional methods of 30 to 40 days.
2. Preparation of culture materials Bacteria that have been cultivated with other types of edible fungi were individually broken, dried and weighed. Add 30% of sawdust or grass clippings by dry weight, corn mixed powder (crush the corn into a soy bean sized mixture of skin, flour, and granules) 10%, dried cow dung or dried chicken manure 6%, lime powder 2%, grass ash 3 %, gypsum powder 1%, fixed nitrogen fertilizer 0.2%. Add water to mix and mix to make the moisture content reach 65%, mix well and then pile up and ferment, turn in the middle 2 or 3 times.
3. Loading inoculation 1 Raw material inoculation: The fermented culture materials are bagged in accordance with the conventional method. Each nut is inserted into the middle of the bag and the whole nutrient-enriched nitrogen type EC05 Coprinus comatus cultivar is 40-50 g; 2 Clinker inoculation: Conventional bagging, sterilization in space sterilization bag for 4 to 5 hours or sterilization in atmospheric pressure sterilization pot for 8 to 12 hours, after the removal of a new open-type edible mushroom sterile inoculator full open bag For oral inoculation, 40 to 50 grams of full-nutrition, high-nitrogen EC05 Coprinus comatus cultivars are inserted at each end of each bag. After the connection, set the clean room, shading and culture to the full bag of mycelium.
4. Fungi bed mushrooming will be covered with mycelium bags upright discharge in the mushroom field ground bacteria bed, open the bag mouth straight tube film, cover the soil surface of the bag material soil 2 ~ 3 cm thick, spray water Wet cover soil, cover the surface of the film layer 1 newspaper, spray water, so that the air relative humidity of 85 to 95%, the first tide mushrooms harvest. After harvesting, the bag will be reversed, and a mouthful of bags will be raised upwards. The bed will be re-arranged, the bag will be opened, the soil will be covered, and the mushroom will be harvested until it is finished. After both ends of the mushroom bag are harvested, the bag tube is removed, the fungus bar is cut vertically, and the cutting plane faces upwards to form a bed of bacteria. The bed surface is covered with fertilizer soil 2 to 3 cm thick, and the water spray covers the soil, and the cover covers the newspaper or cover. The arch membrane cultured mushroom, but also mushroom 2 to 3 tide, the total bioconversion rate of about 250%.
5. The most prominent feature of timely harvesting of EC05 coprinus comatus is coarse, anti-hybrid, anti-aging, mycelium is not clay, and the mycelia in the soil after covering with soil can change over winter or summer with the weather and seasonal changes, when the temperature is appropriate. (8 ~ 32 °C) natural recovery of vitality, broken down soil mushroom. There are no pests during the growing season. When the fruiting body is elongated upwards to 20-30 cm, the strained bacteria strains of the strained cap, and the fungus ring are loosened and unworn, the umbrella is harvested in time, and the edge of the cap is carefully removed from the stipe, opened the umbrella, or dissolved. Become dark and lose the value of goods. When harvesting, the mushroom body is pulled up by hand and pulled up, and the mushroom soil is removed by cutting with a knife, and then it can be sold on the market or processed into a salt product for sale.

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