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Introduction to fumigation of biosafety cabinets
Biosafety cabinets are very familiar to the vast number of research and experimental personnel, but their use and maintenance, especially under the specific conditions of complete fumigation and disinfection is not necessarily well understood, due to many reasons, most laboratories After the biosafety cabinet has been put into use, even for many years, it has not undergone necessary maintenance, and some have even been used in an abnormal state. This leaves a hidden danger to the laboratory biosafety, especially in some specific situations, such as the need to replace the exhaust high-efficiency filter after 3 to 5 years of use, the biosafety cabinet is seriously polluted before the safety cabinet fails and needs to be opened for maintenance. Thorough fumigation is required.
Commonly used disinfectants for fumigation include formaldehyde, hydrogen peroxide, chlorine dioxide, etc. Although formaldehyde is the least expensive, its carcinogenic effects have been increasingly restricted by use, and most people who have used formaldehyde fumigation have headaches. Because the entire safety cabinet is sealed with plastic cloth, the amount of disinfectant required, the placement of biological indicators, neutralizing disinfectant, waiting for a long time, etc., the most worrying is the leakage of formaldehyde and the bio-challenge experiment. Security issues such as success or failure.
There is now a new way to completely replace the traditional formaldehyde fumigation, which uses hydrogen peroxide vapor to sterilize the biosafety cabinet. Usually the generator is connected to the main cabin through a jetting interface. Some models of safety cabinets already provide this interface. At the same time, the door sealing plate seals the front operation port. If neither of these configurations are available, a temporary door seal can be used and the appropriate connection interface is pre-installed, mounted on the front operating port, and hydrogen peroxide vapor is injected through the interface on the door seal. Inside the biosafety cabinet.
Usually the steam circuit is connected to the rear of the safety cabinet exhaust filter, of course the exact type and location of the connection depends on the type of biosafety cabinet with disinfection. When replacing the filter or performing maintenance on the safety cabinet, this connection pushes the hydrogen peroxide vapor through the exhaust filter of the safety cabinet and thoroughly sterilizes it.
It is also feasible to disinfect only the main compartment and can be used to kill all microorganisms in the cabin and under the workbench before the next experiment to avoid cross-contamination. This solution has a shorter sterilization cycle time than the main filter and uses less hydrogen peroxide.
All biosafety cabinets have more or less gas leaks. To ensure process safety, the hydrogen peroxide generator can maintain a small negative pressure condition, ensuring that hydrogen peroxide vapor does not get from the tank during the sterilization process. The body leaked into the laboratory. This function is obtained by attaching a small pressure control tube to the door seal plate before disinfection.