The polarimeter is a more common and important experimental testing instrument in the laboratory. In order to better use and maintain the polarimeter, this article will tell you how to use the automatic polarimeter and maintenance techniques to reduce misuse and instrument failure and extend the life of the instrument. First, the polarimeter structural parts and accessories <br> Consumables: 1. Light source 2. Frosted glass 3. Condenser 4. Filter color 5. Polarizer 6. Half wave plate 7. Test tube 8. Error lens 9. Object, eyepiece group 10 Focusing hand wheel 11. Reading magnifier 12. Dial and cursor 13. Dial turning hand wheel When the test tube is placed in the test tube containing the solution to be tested, the plane polarized light is rotated because the solution has optical rotation. At the angle, the zero field of view changes, turning the analyzer at a certain angle, and the field of view with the same brightness can appear again. This angle is the optical rotation of the solution. After measuring the optical rotation of the solution, the specific rotation of the substance can be determined. According to the specific rotation degree, the purity and content of the substance can be determined. Second, the use of polarimeter: Third, the maintenance of the polarimeter
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(1) Connect the polarimeter to a 220V AC power supply. Turn on the power switch, and after about 5 minutes, the sodium lamp will light up normally and you can start working.
(2) Check whether the zero position of the polarimeter is accurate, that is, when the polarimeter is not placed in the test tube or placed in the test tube filled with distilled water, observe whether the field of view brightness is the same at zero degree. If it is inconsistent, it indicates that there is a zero error, and the deviation value should be subtracted or added to the measurement reading. Or relax the four screws on the back of the disc cover, and correct the disc cover slightly (only correct the error of about 0.5 °, serious should be sent to the factory for inspection).
(3) Select a test tube of appropriate length, fill the test solution, install a rubber band, and screw the nut until it does not leak. The nut should not be screwed too tightly, otherwise the protective glass will cause stress and affect the correctness of the reading. Then, the residual solution at both ends of the test tube is dried to avoid affecting the observation clarity and the measurement accuracy.
(4) Measure the optical rotation reading: rotate the dial, the analyzer, and the position where the brightness is consistent in the field of view, and then read from the dial. The reading is positive for the right-handed substance and the negative for the left-handed substance.
(5) Using the double-cursor reading method, the result can be obtained according to the following formula: Q=(A+B)/2 where: A and B are the reading values ​​of the two cursor windows, respectively. If A=B, and the dial is turned to any position, it means that the polarimeter has no eccentricity (normally, the polarimeter has been calibrated before leaving the factory), and the item reading method can be omitted. (6) Optical rotation and temperature are also related. For most substances, λ = 5893 A (sodium light) was measured, and when the temperature was raised by 1 ° C, the optical rotation was reduced by about 0.3%. For the most demanding measurement work, it is best to carry out at 20 °C ± 2 °C.
(1) The polarimeter should be placed in a well ventilated and well-ventilated place to protect it from moisture.
(2) The continuous use time of the polarimeter should not exceed 4 hours. If the use time is long, the middle should be turned off for 10 to 15 minutes. After the sodium lamp is cooled, continue to use it, or blow it with an electric fan to reduce the degree of heat of the lamp to avoid the decrease of brightness and the decrease of life.
(3) After using the test tube, the solution should be poured out in time, washed with distilled water, and dried. All lenses should not be rubbed directly with your hands and rubbed with a soft flannel.
(4) If there is any problem during the use of the polarimeter, you can contact the southeast instrument sales engineer in time.
(5) When the polarimeter is deactivated, the plastic sleeve should be placed on it. When packing, put it into the box in a fixed position and press it tightly.
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How to use the automatic polarimeter and maintenance techniques
The polarimeter is mainly used to measure the rotation angle and the international standard sugar content. The measurement of the rotation angle allows the quality and safety of the product to be ensured by measuring the specific optical rotation of the chemical, the fragrance chemical raw material, and the like. A polarimeter can be used to determine the angle of rotation or optical rotation of an aqueous sample solution. Specific optical rotation is an intrinsic property of a substance at a specific temperature and wavelength, and can be calculated from the length of the glare tube, the angle of rotation, and the concentration. The international standard sugar content is used to determine the purity of the raw sugar during the sugar process. The sample is measured using the "International Standard Brix" function on the polarimeter. The measured value is the international standard sugar value of the sample. The polarimeter is used for the measurement of optical rotation of pharmaceutical raw materials and finished products in the pharmaceutical industry, the measurement of the components of fine chemical substances in the fine chemical industry or the fragrance, the purity of sugar in the food industry or the quality of invert sugar and the ratio of optically active compounds. Measurement of curl or content.