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Molecular Biotechnology to Realize Germplasm Improvement of Large Yellow Croaker
Molecular Biotechnology to Realize Germplasm Improvement of Large Yellow Croaker On November 23, the project “Planting of Large Yellow Croaker's Chromosome Operation Techniques to Improve Female Breeding and Applied Technology†was jointly undertaken by experts from the Ningde Aquatic Technology Extension Station in Fujian Province and the Fisheries College of Jimei University. Stage acceptance. Experts believe that this technology has made considerable progress and is of great significance to the improvement of the germplasm of large yellow croaker. Through three years of hard work, the project team completed research on artificial gynogenetic induction, sexual hormone-induced gynogenetic fish male cultivating pseudo-male fish, and the development of full-female seedlings by hybridization of pseudo-male fish with normal females. Established techniques for batch breeding of all female diploids of large yellow croaker. Through random sampling, the induction rate of all female diploids of large yellow croaker reached 100%; the induction rate of pseudo males reached 8.5%. Numerous parental groups of different sizes have been bred, including female gynogenesis females, pseudo-male fish, female gynogenesis secondary females, etc., and genetically inherited by crossbreeding with uninverted broodstocks. All the female fry (average length is 3.01cm, average weight is 0.182g) 220,000. At the same time, the project team also made positive progress in basic research, identified the genetic type of sex determination in large yellow croaker, analyzed the genetic variation of wild populations and multi-generation breeding populations in Guanjingyang large yellow croaker, and conducted microsatellites. The separation of markers and application techniques were studied, and the relationship between chromosome ploidy and the number of nucleoli was studied. The expert group for identification said that the application of biotechnology in the research to improve the germplasm of large yellow croaker is of great significance to the realization of improved breeding, the promotion of industrial upgrading, and the improvement of aquaculture production and economic benefits.