At present, enzymatic analysis technology has been widely used in China, such as vegetable, food, juice, dairy, wine, sugar, feed, fermentation, pharmaceutical, condiment and other processing and production as product quality control, finished product quality inspection and The main means of research and analysis of process engineering such as processing and pharmaceuticals, as a means of product quality control and production process monitoring in drug research and development institutions. This is mainly because it has the advantages of simple operation, fast and accurate, high specificity, and the ability to quantitatively analyze and detect. It has been recognized by many countries in the world and has been written into regulations by many countries.
With the rapid development of science and technology, a liquid-packed enzymatic assay kit has been introduced on the market. Compared with the conventional dry powder enzymatic kit, this kit has the following remarkable features: The reagents are all liquid dosage forms, ready to use, do not need to be dissolved and formulated, and overcome some shortcomings of the previous generation of ordinary dry powder kits: for example, some reagents (such as enzyme or NADP) are lyophilized powder, which should be dissolved before use. For cryopreservation, especially the shelf life is only 2-3 months, before each sample is tested, it should be melted, which not only reduces the activity of the enzyme, but also affects the enzymatic reaction and detection accuracy; introduces a new generation of liquid ready-to-use type The kit does not have these problems, and the core enzyme is also liquid type. It can be used directly without cryopreservation. The shelf life is over 12 months, the stability and repeatability of the test are greatly improved. The third is the liquid ready-to-use kit. The instrument can be used for automatic detection, which effectively reduces the amount of reagents, increases the number of tests, and doubles the cost of testing. The above characteristics make the liquid enzymatic analysis kit replace the ordinary dry powder kit with an irreversible advantage.
With the standardization of enzymatic applications, enzymatic analysis has become particularly important in international trade. In China, the current standard level is generally low, so it is necessary to learn from the internationally mature standard system. The Agreement on Technical Barriers to Trade clearly states that wherever technical rules or standards are required, Parties should use these international technical rules or standards as the basis for the development of national technical rules or standards. This is also the need for our exports.

In the following international standards and authoritative regulations, a large number of enzymatic detection methods have been adopted and promoted:
ISO (International Standards Organization)